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Precipitation now (radar) Stringa Lucas SPIRITUAL GROWTH, EDUCATION & WORSHIP Handcrafted, Wood-Fired, Neapolitan Pizza and Italian Shared Plates WILD ROSE FLORALE "As you can see it has a little of the attributes of a mill style building, the exposed wood ceiling, large timbers, brick walls.  We really wanted to bring a little bit of Brooklyn to Salem and I feel like this building has everything that we were looking for."  SLOGGI SWIM BRIGHT FANTASY Reggiseno bikini con ferretto Shenanigans Zappos DPReview Pink Sugar Brooklyn Brown Ale, NY   Gift Card Marrone Glycerol-3-phosphate acyltransferases (GPAT) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of this study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hair-pin RNA targeting Gpat2 mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in Gpat2-silenced germ cells. 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